Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 191526..192061 Replicon plasmid unnamed1
Accession NZ_CP071930
Organism Enterococcus faecium strain VRE3355

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag J5F47_RS01410 Protein ID WP_002305108.1
Coordinates 191789..192061 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag J5F47_RS01405 Protein ID WP_002303154.1
Coordinates 191526..191789 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5F47_RS01380 187529..187801 + 273 WP_002305107.1 DUF3884 family protein -
J5F47_RS01385 187822..188721 + 900 WP_002303235.1 aldose 1-epimerase family protein -
J5F47_RS01395 190435..190998 + 564 Protein_204 IS6 family transposase -
J5F47_RS01400 191004..191420 + 417 WP_002303153.1 hypothetical protein -
J5F47_RS01405 191526..191789 + 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J5F47_RS01410 191789..192061 + 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
J5F47_RS01415 192138..192467 + 330 WP_002292678.1 AbrB family transcriptional regulator -
J5F47_RS01420 192457..192744 + 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
J5F47_RS01425 193030..193887 + 858 WP_002292680.1 AAA family ATPase -
J5F47_RS01430 193888..194436 + 549 WP_002292681.1 hypothetical protein -
J5F47_RS01435 194733..195680 - 948 WP_002303155.1 DNA-binding transcriptional regulator -
J5F47_RS01440 195869..197056 + 1188 WP_002303156.1 NADP-dependent malic enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..220241 220241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T197140 WP_002305108.1 NZ_CP071930:191789-192061 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T197140 NZ_CP095749:3662232-3662495 [Streptomyces yunnanensis]
GTGAGTGAGTACCGCACCGTCTTCCGGCCCGAGGCTCAGGCCGAACTCCGCAAGGTGCCGCGAGACATGGCCCTCCGCAT
TCTGGCCAAGTTGACCGAGCTGGAGAGTGACCCTCTGGGGTTCAACACCACCGCCCTCGTCTCCCAACCCGACCGCCGCC
GCCTACGCGTGGGCGACTACCGCGTGATCTACACGATCGACAACGGCGAGCTGGTGGTGTGGGTCGTGCATGTCGGGCAT
CGCTCCACTGTGTACGACACCTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT197140 WP_002303154.1 NZ_CP071930:191526-191789 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT197140 NZ_CP095749:3661970-3662239 [Streptomyces yunnanensis]
ATGACACAGCCGCTGCCCATAGAGTCCATCCGCGATGTCCGGGCGCACCTGGCCGAGGTCGTGGAACGAGCCGACCGCGA
CGACGTGCCGACGGTAATCACGCGCCGAGGCAAGGAGGTGGCTGCCGTGGTGTCCATCGACGTGCTCCGGAAATACCAGG
AGTGGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGCATGGCCAATCCGGCGCCCGGTATCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCAGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References