Detailed information of TA system TA197132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1311833..1312485
• Replicon: plasmid unnamed
• Accession: NZ_CP071915
• Organism: Ralstonia solanacearum strain SY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: J4H89_RS22415
• Protein ID: WP_028861315.1
• Coordinates: 1311833..1312177 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4H89_RS22420
• Protein ID: WP_020425351.1
• Coordinates: 1312174..1312485 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4H89_RS22400 (J4H89_22395)	1307528..1308691	-	1164	WP_011003438.1	2-methylcitrate synthase	-
J4H89_RS22405 (J4H89_22400)	1308743..1309639	-	897	WP_071092368.1	methylisocitrate lyase	-
J4H89_RS22410 (J4H89_22405)	1309833..1311782	+	1950	WP_214372458.1	propionate catabolism operon regulatory protein PrpR	-
J4H89_RS22415 (J4H89_22410)	1311833..1312177	+	345	WP_028861315.1	hypothetical protein	Toxin
J4H89_RS22420 (J4H89_22415)	1312174..1312485	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
J4H89_RS22425 (J4H89_22420)	1312625..1313434	-	810	WP_029240571.1	DUF2242 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / htpB / adeG / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI	1..2177065	2177065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T197132 WP_028861315.1 NZ_CP071915:1311833-1312177 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T197132 NZ_CP095741:c2575613-2575350 [Luteibacter aegosomaticola]
ATGGCACGGAAGCTCACATTTACGTCAAAAGGCTGGGCCGACTACGCGTCCTGGGCAGACGATCGCAAGATGCTCGCCCG
CGTGAACCTGCTAATCAGGGAGTGCCTGCGCACGCCCACTGAGGGCCTGGCCAAACCAAAGCCGATGCGCGGCGATCTTG
CCGGCGCCTGGTCGCGGCGCATCGATGAGAGGCACCGCCTGGTCTATCTGTTCACCGACCACGAACTCGAAGTGATCGCG
TGCCGCCTGCATACGACGGAGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT197132 WP_020425351.1 NZ_CP071915:1312174-1312485 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT197132 NZ_CP095741:c2575886-2575620 [Luteibacter aegosomaticola]
ATGCGCGCTATCAACTATTCCGAGGTCCGGAACAACCTCGCGACCGTCCTTGATACGGTTGTGGACGATGCCGAAGTGGC
GGTGATCACGCGCCGCGGCGACGCCGACGGCAGCCGGGCGGTCTACCTCGTTCCTGCGCGGCTGTGGGAAAGCATGGAGG
AAACCGCCTACCTTCTCTCCGGCGCGAACCGCGGCCACATCCTCGAAAGCCTGCGCCAGGCGCGGGCGGGCGAAGTGTTC
GAGCGGCCGTTGATTGAAGACGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4