Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1194303..1195030 Replicon chromosome
Accession NZ_CP071876
Organism Enterobacter hormaechei strain GX4-8L

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag J4O68_RS05725 Protein ID WP_022650547.1
Coordinates 1194303..1194614 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag J4O68_RS05730 Protein ID WP_022650546.1
Coordinates 1194611..1195030 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4O68_RS05705 (J4O68_05700) 1190575..1191354 - 780 WP_026094386.1 acetolactate decarboxylase -
J4O68_RS05710 (J4O68_05705) 1191460..1192341 + 882 WP_032670419.1 LysR family transcriptional regulator -
J4O68_RS05715 (J4O68_05710) 1192623..1193078 + 456 WP_032657484.1 hypothetical protein -
J4O68_RS05720 1193191..1193847 - 657 WP_022650548.1 hypothetical protein -
J4O68_RS05725 (J4O68_05720) 1194303..1194614 + 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
J4O68_RS05730 (J4O68_05725) 1194611..1195030 + 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
J4O68_RS05735 (J4O68_05730) 1195354..1195788 + 435 WP_045342299.1 hypothetical protein -
J4O68_RS05740 (J4O68_05735) 1195841..1196731 - 891 WP_045342293.1 oxidoreductase -
J4O68_RS05745 (J4O68_05740) 1196837..1197958 + 1122 WP_003858868.1 YbdK family carboxylate-amine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T197099 WP_022650547.1 NZ_CP071876:1194303-1194614 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T197099 NZ_CP095556:c3429875-3429594 [Pseudomonas mosselii]
ATGACCTATAGGCTCGAGTTCCTGCCTTCGGCCCTGAAGGAGTGGGAGAAGCTGGGGCACACCGTGCGTGAGCAGGCCAG
AAAGAAGCTCCGGCAACGCTTGCTCTCGCCCAAGGTCGGTGTGGATGCGCTGCGTGATTTGCCCAACCACTACAAGATCA
AGCTCAGGGCCAGCGGCTATCGACTGGTCTACCGCGTCGAGGATGATCGGGTAGTGGTGGTGGTGGTGGCGATAGGCAAG
CGTGAGCGCGGGCAGGCCTACCGCTCGGCTTACACTCGCTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT197099 WP_022650546.1 NZ_CP071876:1194611-1195030 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT197099 NZ_CP095556:c3430110-3429865 [Pseudomonas mosselii]
ATGCAGAACATCTTTGCCGACGTCACTGTCAGTGTCTCGGAATTAAAGAAGAATCCTACGGCAGTCATGGCCGATGCACA
GGGGAAACCGGTGGCAGTCCTCAACCATAATCGCGTCATGGGCTACATGGTGCCCGCTGAACTCTACGAGGCGATGATGG
AACGACTGGAAGATATCGAACTGGTGGCATTGGTCAAGGCGCGCGCGGGAGAGAAAGGCATCCCGGTCGAGCTGGATGAC
CTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References