Detailed information of TA system TA197071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2179922..2180571
• Replicon: chromosome
• Accession: NZ_CP071861
• Organism: Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1A383
• Locus tag: J4G41_RS10480
• Protein ID: WP_013097692.1
• Coordinates: 2180209..2180571 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4G41_RS10475
• Protein ID: WP_058681148.1
• Coordinates: 2179922..2180221 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G41_RS10465 (J4G41_10465)	2176334..2178307	+	1974	WP_058681149.1	PhoX family phosphatase	-
J4G41_RS10470 (J4G41_10470)	2178410..2179798	+	1389	WP_023620421.1	phenylalanine transporter	-
J4G41_RS10475 (J4G41_10475)	2179922..2180221	-	300	WP_058681148.1	helix-turn-helix transcriptional regulator	Antitoxin
J4G41_RS10480 (J4G41_10480)	2180209..2180571	-	363	WP_013097692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G41_RS10485 (J4G41_10485)	2180776..2182014	+	1239	WP_029882613.1	MFS transporter	-
J4G41_RS10490 (J4G41_10490)	2182030..2183055	+	1026	WP_058681147.1	sugar phosphate isomerase/epimerase	-
J4G41_RS10495 (J4G41_10495)	2183048..2184190	+	1143	WP_058683110.1	Gfo/Idh/MocA family oxidoreductase	-
J4G41_RS10500 (J4G41_10500)	2184266..2185237	+	972	WP_058681145.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13994.00 Da
• Isoelectric Point: 7.3235

>T197071 WP_013097692.1 NZ_CP071861:c2180571-2180209 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Nucleotide

• Length: 363 bp

>T197071 NZ_CP095538:c4364677-4364504 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.65 Da
• Isoelectric Point: 8.6152

>AT197071 WP_058681148.1 NZ_CP071861:c2180221-2179922 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIELPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Nucleotide

• Length: 300 bp

>AT197071 NZ_CP095538:c4365001-4364705 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A5E1A383