Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 924827..925557 Replicon chromosome
Accession NZ_CP071861
Organism Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag J4G41_RS04465 Protein ID WP_058681570.1
Coordinates 925243..925557 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J4G41_RS04460 Protein ID WP_167816673.1
Coordinates 924827..925246 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS04435 (J4G41_04450) 920318..921823 - 1506 WP_058682155.1 signal transduction histidine-protein kinase/phosphatase UhpB -
J4G41_RS04440 (J4G41_04455) 921820..922413 - 594 WP_013094809.1 transcriptional regulator UhpA -
J4G41_RS04445 (J4G41_04460) 922496..922783 - 288 WP_001171659.1 acetolactate synthase small subunit -
J4G41_RS04450 (J4G41_04465) 922787..924475 - 1689 WP_046888828.1 acetolactate synthase large subunit -
J4G41_RS04455 (J4G41_04470) 924582..924680 - 99 WP_071830013.1 ilvB operon leader peptide IvbL -
J4G41_RS04460 (J4G41_04475) 924827..925246 - 420 WP_167816673.1 helix-turn-helix domain-containing protein Antitoxin
J4G41_RS04465 (J4G41_04480) 925243..925557 - 315 WP_058681570.1 type II toxin-antitoxin system HigB family toxin Toxin
J4G41_RS04470 (J4G41_04485) 926046..926135 + 90 WP_020684667.1 type I toxin-antitoxin system toxin TisB -
J4G41_RS04475 (J4G41_04490) 926200..926646 + 447 WP_058682154.1 GNAT family N-acetyltransferase -
J4G41_RS04480 (J4G41_04495) 926709..927542 + 834 WP_058682153.1 EamA family transporter -
J4G41_RS04485 (J4G41_04500) 927718..928902 + 1185 WP_014830051.1 multidrug efflux MFS transporter EmrD -
J4G41_RS04490 (J4G41_04505) 928805..929893 - 1089 WP_058682152.1 cellulase family glycosylhydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(1-139)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12338.55 Da        Isoelectric Point: 10.2219

>T197066 WP_058681570.1 NZ_CP071861:c925557-925243 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLVLGRTLEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANKNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHLTKGKK

Download         Length: 315 bp

>T197066 NZ_CP095538:3482510-3482728 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15581.19 Da        Isoelectric Point: 4.9597

>AT197066 WP_167816673.1 NZ_CP071861:c925246-924827 [Enterobacter cloacae]
MMIVADAMKATHALVEAVPLLGEQPSEKDYNDALELVEYLLINEPNSPLLDIVCARISRYEANRPEIVALRKEMELVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLASRFGVSPALFIE

Download         Length: 420 bp

>AT197066 NZ_CP095538:3482110-3482484 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References