Detailed information of TA system TA197012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1338902..1339507
• Replicon: chromosome
• Accession: NZ_CP071826
• Organism: Burkholderia anthina strain 1CH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J4G50_RS25055
• Protein ID: WP_096498818.1
• Coordinates: 1339229..1339507 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2A4CLK3
• Locus tag: J4G50_RS25050
• Protein ID: WP_057930197.1
• Coordinates: 1338902..1339216 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G50_RS25040	1336158..1336922	-	765	WP_208000375.1	helix-turn-helix domain-containing protein	-
J4G50_RS25045	1337139..1338791	+	1653	WP_208000376.1	AMP-binding protein	-
J4G50_RS25050	1338902..1339216	-	315	WP_057930197.1	HigA family addiction module antidote protein	Antitoxin
J4G50_RS25055	1339229..1339507	-	279	WP_096498818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G50_RS25060	1339601..1340032	-	432	WP_208000377.1	VTT domain-containing protein	-
J4G50_RS25065	1340141..1340785	-	645	WP_208000378.1	LysE family transporter	-
J4G50_RS25070	1341072..1341554	+	483	WP_096498814.1	Lrp/AsnC family transcriptional regulator	-
J4G50_RS25075	1342364..1344016	-	1653	WP_208000379.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-98)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10484.85 Da
• Isoelectric Point: 7.7175

>T197012 WP_096498818.1 NZ_CP071826:c1339507-1339229 [Burkholderia anthina]
MIQSFKCKDTRQLFETGRCRQFANIADVAMRRLTQLDNAATLDAMKSPPGNRFEALKGDRVGQYSIRINGQWRVCFTWGD
DGPADVEIADYH

Nucleotide

• Length: 279 bp

>T197012 NZ_CP095529:577948-578298 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11785.46 Da
• Isoelectric Point: 9.6536

>AT197012 WP_057930197.1 NZ_CP071826:c1339216-1338902 [Burkholderia anthina]
MTRFKNGMRPVHPGEILREDYLIPLDMSANALAGALGVTAGRINDIVRERRGITADTALRLARYFGGDPQTWLNLQATYD
LKIAERDHGREIQKRIEPREFAHA

Nucleotide

• Length: 315 bp

>AT197012 NZ_CP095529:577703-577954 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATTTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A4CLK3