Detailed information of TA system TA197002

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58106..58370
• Replicon: plasmid p90CM2-103k
• Accession: NZ_CP071822
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: IM758_RS28255
• Protein ID: WP_024179505.1
• Coordinates: 58218..58370 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	58106..58166 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM758_RS28240 (53345)	53345..55636	-	2292	WP_077265944.1	F-type conjugative transfer protein TrbC	-
IM758_RS28245 (55629)	55629..56699	-	1071	WP_064148470.1	IncI1-type conjugal transfer protein TrbB	-
IM758_RS28250 (56718)	56718..57926	-	1209	WP_064148469.1	IncI1-type conjugal transfer protein TrbA	-
- (58106)	58106..58166	-	61	NuclAT_0	-	Antitoxin
- (58106)	58106..58166	-	61	NuclAT_0	-	Antitoxin
- (58106)	58106..58166	-	61	NuclAT_0	-	Antitoxin
- (58106)	58106..58166	-	61	NuclAT_0	-	Antitoxin
IM758_RS28255 (58218)	58218..58370	+	153	WP_024179505.1	Hok/Gef family protein	Toxin
IM758_RS28260 (58442)	58442..58693	-	252	WP_001291962.1	hypothetical protein	-
- (59086)	59086..59137	-	52	NuclAT_1	-	-
- (59086)	59086..59137	-	52	NuclAT_1	-	-
- (59086)	59086..59137	-	52	NuclAT_1	-	-
- (59086)	59086..59137	-	52	NuclAT_1	-	-
IM758_RS28265 (60026)	60026..60202	-	177	WP_180335155.1	hypothetical protein	-
IM758_RS28270 (60595)	60595..60804	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
IM758_RS28275 (60876)	60876..61538	-	663	WP_060527638.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103107	103107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5743.07 Da
• Isoelectric Point: 8.7948

>T197002 WP_024179505.1 NZ_CP071822:58218-58370 [Klebsiella pneumoniae subsp. pneumoniae]
MPQRTFLMMLIVVCVTILCFVWIVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T197002 NZ_CP095526:c3680431-3680258 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT197002 NZ_CP071822:c58166-58106 [Klebsiella pneumoniae subsp. pneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin