Detailed information of TA system TA196966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 9083..9748
• Replicon: plasmid pYRKMM821_2
• Accession: NZ_CP071804
• Organism: Yersinia ruckeri strain KMM821

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: YR821_RS17220
• Protein ID: WP_038251855.1
• Coordinates: 9425..9748 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: YR821_RS17215
• Protein ID: WP_004720859.1
• Coordinates: 9083..9379 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YR821_RS17195 (YR821_p20003)	4429..6354	-	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-
YR821_RS17200 (YR821_p20004)	6356..6700	-	345	WP_004720848.1	plasmid mobilization protein MobA	-
YR821_RS17205 (YR821_p20005)	6969..7292	+	324	WP_004720850.1	hypothetical protein	-
YR821_RS17210 (YR821_p20007)	7831..8649	-	819	WP_004720855.1	DUF932 domain-containing protein	-
YR821_RS17215 (YR821_p20009)	9083..9379	-	297	WP_004720859.1	NadS family protein	Antitoxin
YR821_RS17220 (YR821_p20010)	9425..9748	-	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YR821_RS17225 (YR821_p20011)	9900..10133	+	234	WP_038251853.1	hypothetical protein	-
YR821_RS17230 (YR821_p20012)	10141..10515	-	375	WP_004720865.1	hypothetical protein	-
YR821_RS17235 (YR821_p20013)	10604..11299	-	696	WP_038251906.1	IS6 family transposase	-
YR821_RS17240 (YR821_p20015)	11840..13039	+	1200	WP_038251850.1	AAA family ATPase	-
YR821_RS17245 (YR821_p20016)	13048..14016	+	969	WP_004723386.1	ParB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103906	103906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T196966 WP_038251855.1 NZ_CP071804:c9748-9425 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T196966 NZ_CP095520:197813-198163 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT196966 WP_004720859.1 NZ_CP071804:c9379-9083 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT196966 NZ_CP095520:197568-197819 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A380S9H4