Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5817423..5818003 Replicon chromosome
Accession NZ_CP071797
Organism Pseudomonas fluorescens strain YK-310

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag JZM58_RS25895 Protein ID WP_207983993.1
Coordinates 5817423..5817701 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JZM58_RS25900 Protein ID WP_045121630.1
Coordinates 5817716..5818003 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZM58_RS25875 (JZM58_25875) 5813005..5813172 - 168 WP_007950992.1 DUF2474 domain-containing protein -
JZM58_RS25880 (JZM58_25880) 5813230..5814237 - 1008 WP_085608716.1 cytochrome d ubiquinol oxidase subunit II -
JZM58_RS25885 (JZM58_25885) 5814241..5815677 - 1437 WP_207983992.1 cytochrome ubiquinol oxidase subunit I -
JZM58_RS25890 (JZM58_25890) 5816078..5817316 + 1239 WP_011336035.1 MFS transporter -
JZM58_RS25895 (JZM58_25895) 5817423..5817701 + 279 WP_207983993.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JZM58_RS25900 (JZM58_25900) 5817716..5818003 + 288 WP_045121630.1 HigA family addiction module antitoxin Antitoxin
JZM58_RS25905 (JZM58_25905) 5818025..5818561 - 537 Protein_5109 DJ-1/PfpI family protein -
JZM58_RS25910 (JZM58_25910) 5818629..5819078 - 450 WP_207983994.1 DUF4879 domain-containing protein -
JZM58_RS25915 (JZM58_25915) 5819299..5820594 + 1296 WP_127800026.1 NCS2 family permease -
JZM58_RS25920 (JZM58_25920) 5820591..5821670 + 1080 WP_207983995.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
JZM58_RS25925 (JZM58_25925) 5821726..5822172 - 447 WP_207983996.1 hypothetical protein -
JZM58_RS25930 (JZM58_25930) 5822169..5822597 - 429 WP_207983997.1 DUF4160 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-75)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10701.27 Da        Isoelectric Point: 8.9401

>T196950 WP_207983993.1 NZ_CP071797:5817423-5817701 [Pseudomonas fluorescens]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDRAAVPGDLDLPGWRLHPLKGELCEYWSLSVSGNWRVIFRFAG
SDIELIDYLDYH

Download         Length: 279 bp

>T196950 NZ_CP095518:c1654976-1654803 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10542.17 Da        Isoelectric Point: 6.9219

>AT196950 WP_045121630.1 NZ_CP071797:5817716-5818003 [Pseudomonas fluorescens]
MPMHNPPHPGETLLMDVLPELGISVTELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EHREQPAIQPIAAHA

Download         Length: 288 bp

>AT196950 NZ_CP095518:c1655300-1655004 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References