Detailed information of TA system TA196923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 330534..331012
• Replicon: chromosome
• Accession: NZ_CP071785
• Organism: Marinobacter sp. CA1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J2887_RS01470
• Protein ID: WP_267461609.1
• Coordinates: 330534..330719 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J2887_RS01475
• Protein ID: WP_227173649.1
• Coordinates: 330719..331012 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2887_RS01435 (J2887_01425)	325860..326303	+	444	WP_227173635.1	antibiotic biosynthesis monooxygenase	-
J2887_RS01440 (J2887_01430)	326323..327789	+	1467	WP_227173637.1	protein kinase	-
J2887_RS01445 (J2887_01435)	327863..328834	+	972	WP_227173639.1	hypothetical protein	-
J2887_RS01455 (J2887_01445)	329214..329450	-	237	WP_227173644.1	addiction module protein	-
J2887_RS21650 (J2887_01450)	329647..329925	+	279	Protein_287	type II toxin-antitoxin system RelE/ParE family toxin	-
J2887_RS01465 (J2887_01455)	329936..330250	+	315	WP_227173647.1	HigA family addiction module antitoxin	-
J2887_RS01470 (J2887_01460)	330534..330719	+	186	WP_267461609.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J2887_RS01475 (J2887_01465)	330719..331012	+	294	WP_227173649.1	HigA family addiction module antitoxin	Antitoxin
J2887_RS01480 (J2887_01470)	331354..332493	-	1140	WP_227173627.1	IS110 family transposase	-
J2887_RS01485 (J2887_01475)	332625..333203	+	579	WP_227173650.1	plasmid pRiA4b ORF-3 family protein	-
J2887_RS01490 (J2887_01480)	333434..333751	-	318	WP_227173652.1	CcdB family protein	-
J2887_RS01495 (J2887_01485)	333751..333996	-	246	WP_227173654.1	type II toxin-antitoxin system CcdA family antitoxin	-
J2887_RS01500 (J2887_01490)	334130..334531	-	402	WP_227173656.1	PIN domain nuclease	-
J2887_RS01505 (J2887_01495)	334633..334887	-	255	WP_227173658.1	Txe/YoeB family addiction module toxin	-
J2887_RS01510 (J2887_01500)	334884..335135	-	252	WP_227173659.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
J2887_RS01515 (J2887_01505)	335328..335693	-	366	WP_227173661.1	histidine triad nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flgI / lfgG / flgC / lfiI / lfhA / fliP	262529..643278	380749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-61)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7174.03 Da
• Isoelectric Point: 4.5899

>T196923 WP_267461609.1 NZ_CP071785:330534-330719 [Marinobacter sp. CA1]
MQLAALDTATSVEDMDIPGFRLYPLKARDKGRWSIRVTGNWRMTFEFQDGNAYIPDYEDYH

Nucleotide

• Length: 186 bp

>T196923 NZ_CP095514:c885645-885493 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10839.35 Da
• Isoelectric Point: 5.0359

>AT196923 WP_227173649.1 NZ_CP071785:330719-331012 [Marinobacter sp. CA1]
MAMHNPPHPGEFIREVYLEPFDISSRQLASSLGVSPSTLSRLLKGNSGISPEMSLRLSKVLGRSPESWLAMQDMYDLWVA
RNTVNLDGIHPLDFEEA

Nucleotide

• Length: 294 bp

>AT196923 NZ_CP095514:885694-885751 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA