Detailed information of TA system TA196891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 164815..165400
• Replicon: plasmid pGX5
• Accession: NZ_CP071769
• Organism: Acinetobacter towneri strain GX5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: J4G45_RS00830
• Protein ID: WP_000897307.1
• Coordinates: 165080..165400 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4G45_RS00825
• Protein ID: WP_000369781.1
• Coordinates: 164815..165087 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G45_RS00805 (J4G45_00800)	160031..160642	+	612	WP_015060246.1	recombinase family protein	-
J4G45_RS00810 (J4G45_00805)	160832..161716	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
J4G45_RS00815 (J4G45_00810)	161772..163247	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
J4G45_RS00820 (J4G45_00815)	163745..164704	+	960	WP_180006246.1	IS30 family transposase	-
J4G45_RS00825 (J4G45_00820)	164815..165087	-	273	WP_000369781.1	NadS family protein	Antitoxin
J4G45_RS00830 (J4G45_00825)	165080..165400	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G45_RS00835 (J4G45_00830)	165540..165959	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
J4G45_RS00840 (J4G45_00835)	166110..167435	+	1326	WP_096902756.1	ISNCY family transposase	-
J4G45_RS00845 (J4G45_00840)	167526..167807	-	282	WP_000985609.1	putative addiction module antidote protein	-
J4G45_RS00850 (J4G45_00845)	167808..168110	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J4G45_RS00855 (J4G45_00850)	168259..168789	+	531	WP_000172759.1	hypothetical protein	-
J4G45_RS00860 (J4G45_00855)	168878..169192	-	315	WP_004728120.1	BrnA antitoxin family protein	-
J4G45_RS13675	169179..169372	-	194	Protein_174	BrnT family toxin	-
J4G45_RS00865 (J4G45_00860)	169351..169728	-	378	Protein_175	AraC family ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / dfrA20 / tet(X3) / aph(3')-Ia / mph(E) / msr(E) / blaOXA-58	-	1..178808	178808
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	145341..173460	28119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T196891 WP_000897307.1 NZ_CP071769:c165400-165080 [Acinetobacter towneri]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T196891 NZ_CP095505:c4073286-4073068 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT196891 WP_000369781.1 NZ_CP071769:c165087-164815 [Acinetobacter towneri]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT196891 NZ_CP095505:c4073686-4073312 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3