Detailed information of TA system TA196887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5160..5745
• Replicon: plasmid pGX3-2
• Accession: NZ_CP071768
• Organism: Acinetobacter towneri strain GX3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: J4G44_RS13280
• Protein ID: WP_000897307.1
• Coordinates: 5425..5745 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4G44_RS13275
• Protein ID: WP_000369781.1
• Coordinates: 5160..5432 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G44_RS13250 (J4G44_13250)	426..848	-	423	WP_004676061.1	hypothetical protein	-
J4G44_RS13255 (J4G44_13255)	952..1275	+	324	WP_004676058.1	hypothetical protein	-
J4G44_RS13260 (J4G44_13260)	1451..2062	+	612	WP_015060246.1	recombinase family protein	-
J4G44_RS13265 (J4G44_13265)	2252..3136	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
J4G44_RS13270 (J4G44_13270)	3192..4667	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
J4G44_RS13275 (J4G44_13275)	5160..5432	-	273	WP_000369781.1	NadS family protein	Antitoxin
J4G44_RS13280 (J4G44_13280)	5425..5745	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G44_RS13285 (J4G44_13285)	6103..7671	+	1569	WP_159124549.1	ATP-binding cassette domain-containing protein	-
J4G44_RS13290 (J4G44_13290)	7908..8198	-	291	WP_180153355.1	hypothetical protein	-
J4G44_RS13295 (J4G44_13295)	8198..8839	-	642	WP_180073099.1	AAA family ATPase	-
J4G44_RS13300 (J4G44_13300)	9973..10275	+	303	WP_180153357.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E)	-	1..54135	54135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T196887 WP_000897307.1 NZ_CP071768:c5745-5425 [Acinetobacter towneri]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T196887 NZ_CP095505:3496181-3496555 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT196887 WP_000369781.1 NZ_CP071768:c5432-5160 [Acinetobacter towneri]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT196887 NZ_CP095505:3495723-3496091 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3