Detailed information of TA system TA196862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1429028..1429685
• Replicon: chromosome
• Accession: NZ_CP071759
• Organism: Burkholderia pseudomallei strain VBP21885

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J3J51_RS06570
• Protein ID: WP_187328920.1
• Coordinates: 1429341..1429685 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J3J51_RS06565
• Protein ID: WP_243435429.1
• Coordinates: 1429028..1429303 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3J51_RS06540 (J3J51_06540)	1424505..1424918	-	414	WP_131201524.1	very short patch repair endonuclease	-
J3J51_RS06545 (J3J51_06545)	1425093..1425326	-	234	WP_131201523.1	hypothetical protein	-
J3J51_RS06550 (J3J51_06550)	1425794..1426873	-	1080	WP_151271492.1	DUF3396 domain-containing protein	-
J3J51_RS06555 (J3J51_06555)	1426873..1427586	-	714	WP_207865924.1	VRR-NUC domain-containing protein	-
J3J51_RS06560 (J3J51_06560)	1427583..1428125	-	543	WP_131201532.1	PAAR domain-containing protein	-
J3J51_RS31115	1428615..1428848	-	234	WP_131201521.1	hypothetical protein	-
J3J51_RS06565 (J3J51_06565)	1429028..1429303	-	276	WP_243435429.1	helix-turn-helix transcriptional regulator	Antitoxin
J3J51_RS06570 (J3J51_06570)	1429341..1429685	-	345	WP_187328920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3J51_RS06575 (J3J51_06575)	1429726..1430394	-	669	WP_243435409.1	site-specific integrase	-
J3J51_RS06580 (J3J51_06580)	1430680..1431459	-	780	WP_038799540.1	hypothetical protein	-
J3J51_RS06585 (J3J51_06585)	1431974..1432207	+	234	WP_038726517.1	hypothetical protein	-
J3J51_RS06590 (J3J51_06590)	1432221..1433567	-	1347	WP_038726519.1	type II secretory pathway protein	-
J3J51_RS06595 (J3J51_06595)	1433564..1434490	-	927	WP_011852346.1	zonular occludens toxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1420626..1442555	21929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (29-83)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13104.18 Da
• Isoelectric Point: 10.1007

>T196862 WP_187328920.1 NZ_CP071759:c1429685-1429341 [Burkholderia pseudomallei]
MKQNWTVVYYNERVKRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGDGLFELRPKGREGIGRVLYCTHFGRHVV
VLHSFVKKTQEAPQKELRTARARLSEVRNGESGC

Nucleotide

• Length: 345 bp

>T196862 NZ_CP095481:c10321348-10320950 [Amycolatopsis sp. WQ 127309]
GTGATCATCGACAGCTCGGCCATTGTGGCGATCCTGCGCCAGGAGCCGGACCACGAACGACTCGAGGAGCGCCTCGCGGA
GGCGGACTCGGCCAGGATCGGCGCGCCGACTCTCGTCGAGGTCGGCATCGTGCTCACCACGCGGCTCGGCGCGCGGGGCC
AGCTGGTGCTCGCCAGGTTCCTCCAGGACAACAACATCGCGACCGCACCCTTCACCGAAGAACACGCGGAGGTGGCGATC
GAAGCGTTCAAGCAGTACGGGAAGGGACATCACCCGGCCAAGCTGAACATGGGCGACTGCTACAGCTATGCCACCGCCAA
GGTCGCCCGGGAGCCGTTGCTGTGCATCGGCGACGACTTCCCGCAGACCGATCTCGAGCTGGTGAAGCTCGGGTCGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9806.08 Da
• Isoelectric Point: 8.0016

>AT196862 WP_243435429.1 NZ_CP071759:c1429303-1429028 [Burkholderia pseudomallei]
MTDDTERLLAKPGVKAQYDALEDEYSALRAILTARQDAGLTQAQVAERMGTTASAVSRLEASLSSERHSPSFATLRKYAA
ACGKRLVISFA

Nucleotide

• Length: 276 bp

>AT196862 NZ_CP095481:c10321617-10321345 [Amycolatopsis sp. WQ 127309]
ATGGCGATGAACATCAAGGACCCGGAGGCCGAGCGCTTGGCCGCCGAGGTCGCCGCACTGACGGGTGACACCAAAACCGG
TGCCGTCCGCGAAGCCCTGCGCCTGCGCCGCGACCAGCTCATGGCGCGGGACAACGCCGAAGTGCGGCTGAAGCGGATGC
GGCGAGTACTGGAAGAGGAGATCTGGCCGTTGATCCCGCCTGAGGAGCTCGGGAAGCCGATCCCGAAGGACGAGTGGGAA
GAGATCCTCGGGTTCGGGCCGGGTGGCGTGTGA