Detailed information of TA system TA196853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 11009..11666
• Replicon: chromosome
• Accession: NZ_CP071754
• Organism: Burkholderia pseudomallei strain VBP364

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J3D99_RS00085
• Protein ID: WP_038738757.1
• Coordinates: 11334..11666 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J3D99_RS00080
• Protein ID: WP_038738759.1
• Coordinates: 11009..11341 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3D99_RS00050 (J3D99_05650)	7335..7979	-	645	WP_004195754.1	lytic polysaccharide monooxygenase	-
J3D99_RS00055 (J3D99_05655)	8483..8800	-	318	WP_004266306.1	VF_A0006 family four-cysteine protein	-
J3D99_RS00060 (J3D99_05660)	8975..9241	+	267	WP_223871971.1	hypothetical protein	-
J3D99_RS00070 (J3D99_05670)	9573..10277	-	705	WP_220231846.1	hypothetical protein	-
J3D99_RS00075 (J3D99_05675)	10270..10965	-	696	WP_137984721.1	hypothetical protein	-
J3D99_RS00080 (J3D99_05680)	11009..11341	-	333	WP_038738759.1	helix-turn-helix domain-containing protein	Antitoxin
J3D99_RS00085 (J3D99_05685)	11334..11666	-	333	WP_038738757.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3D99_RS00090 (J3D99_05690)	11708..12532	-	825	WP_258571441.1	site-specific integrase	-
J3D99_RS00095 (J3D99_05695)	12662..13441	-	780	WP_038738756.1	hypothetical protein	-
J3D99_RS00100 (J3D99_05700)	13967..14200	+	234	WP_038726517.1	hypothetical protein	-
J3D99_RS00105 (J3D99_05705)	14214..15560	-	1347	WP_131201517.1	type II secretory pathway protein	-
J3D99_RS00110 (J3D99_05710)	15557..16483	-	927	WP_038757272.1	zonular occludens toxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2531..24548	22017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-102)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12696.69 Da
• Isoelectric Point: 10.4237

>T196853 WP_038738757.1 NZ_CP071754:c11666-11334 [Burkholderia pseudomallei]
MEQNWTVIYYNERVKRDVLSLPAGILADYLRLLDLMREFGADLRMPHSRAMGGGLFELRPKGREGIGRVFYCTHVGRHIV
VLHSFVKKTQETPQNDLRTARARLSEVRNG

Nucleotide

• Length: 333 bp

>T196853 NZ_CP095463:198915-199172 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11832.50 Da
• Isoelectric Point: 10.2749

>AT196853 WP_038738759.1 NZ_CP071754:c11341-11009 [Burkholderia pseudomallei]
MVKAAAKRARAEGFNPVPHSADDTERLLAKRGVRAAYDALEDEYGVLRAILLARQEAGLTQAQVAERMGTTASAVSRLEA
SLSSERHSPSFATLRKYAAACGKRLIISFA

Nucleotide

• Length: 333 bp

>AT196853 NZ_CP095463:198653-198913 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA