Detailed information of TA system TA196806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1581820..1582415
• Replicon: chromosome
• Accession: NZ_CP071731
• Organism: Pseudomonas aeruginosa strain LYSZa2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LYSZa2_RS07570
• Protein ID: WP_003117425.1
• Coordinates: 1582137..1582415 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LYSZa2_RS07565
• Protein ID: WP_003113527.1
• Coordinates: 1581820..1582125 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYSZa2_RS07530 (LYSZa2_07530)	1577295..1577585	-	291	WP_077144418.1	DUF5447 family protein	-
LYSZa2_RS07535 (LYSZa2_07535)	1577589..1577771	-	183	WP_126534041.1	hypothetical protein	-
LYSZa2_RS07540 (LYSZa2_07540)	1577761..1578033	-	273	WP_004352675.1	hypothetical protein	-
LYSZa2_RS07545 (LYSZa2_07545)	1578480..1578815	+	336	WP_077144417.1	hypothetical protein	-
LYSZa2_RS07550 (LYSZa2_07550)	1578895..1579917	-	1023	WP_077144416.1	reverse transcriptase family protein	-
LYSZa2_RS07555 (LYSZa2_07555)	1580177..1580539	-	363	WP_126534043.1	hypothetical protein	-
LYSZa2_RS07560 (LYSZa2_07560)	1580539..1581384	-	846	WP_126534045.1	hypothetical protein	-
LYSZa2_RS07565 (LYSZa2_07565)	1581820..1582125	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LYSZa2_RS07570 (LYSZa2_07570)	1582137..1582415	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LYSZa2_RS30965	1582468..1582596	-	129	Protein_1496	integrase	-
LYSZa2_RS07575 (LYSZa2_07575)	1582744..1584972	+	2229	WP_003113525.1	TonB-dependent receptor	-
LYSZa2_RS07580 (LYSZa2_07580)	1585042..1585689	-	648	WP_003095021.1	carbonate dehydratase	-
LYSZa2_RS07585 (LYSZa2_07585)	1585751..1586989	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T196806 WP_003117425.1 NZ_CP071731:c1582415-1582137 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T196806 NZ_CP095427:c56969-56553 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT196806 WP_003113527.1 NZ_CP071731:c1582125-1581820 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT196806 NZ_CP095427:c57196-56966 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5