Detailed information of TA system TA196799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1581829..1582424
• Replicon: chromosome
• Accession: NZ_CP071730
• Organism: Pseudomonas aeruginosa strain LYSZa5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LYSZa5_RS07570
• Protein ID: WP_003117425.1
• Coordinates: 1582146..1582424 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LYSZa5_RS07565
• Protein ID: WP_003113527.1
• Coordinates: 1581829..1582134 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYSZa5_RS07530 (LYSZa5_07530)	1577304..1577594	-	291	WP_077144418.1	DUF5447 family protein	-
LYSZa5_RS07535 (LYSZa5_07535)	1577598..1577780	-	183	WP_126534041.1	hypothetical protein	-
LYSZa5_RS07540 (LYSZa5_07540)	1577770..1578042	-	273	WP_004352675.1	hypothetical protein	-
LYSZa5_RS07545 (LYSZa5_07545)	1578489..1578824	+	336	WP_077144417.1	hypothetical protein	-
LYSZa5_RS07550 (LYSZa5_07550)	1578904..1579926	-	1023	WP_077144416.1	reverse transcriptase family protein	-
LYSZa5_RS07555 (LYSZa5_07555)	1580186..1580548	-	363	WP_126534043.1	hypothetical protein	-
LYSZa5_RS07560 (LYSZa5_07560)	1580548..1581393	-	846	WP_126534045.1	hypothetical protein	-
LYSZa5_RS07565 (LYSZa5_07565)	1581829..1582134	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LYSZa5_RS07570 (LYSZa5_07570)	1582146..1582424	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LYSZa5_RS30965	1582477..1582605	-	129	Protein_1496	integrase	-
LYSZa5_RS07575 (LYSZa5_07575)	1582753..1584981	+	2229	WP_003113525.1	TonB-dependent receptor	-
LYSZa5_RS07580 (LYSZa5_07580)	1585051..1585698	-	648	WP_003095021.1	carbonate dehydratase	-
LYSZa5_RS07585 (LYSZa5_07585)	1585760..1586998	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T196799 WP_003117425.1 NZ_CP071730:c1582424-1582146 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T196799 NZ_CP095426:c4787061-4786528 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT196799 WP_003113527.1 NZ_CP071730:c1582134-1581829 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT196799 NZ_CP095426:c4787338-4787072 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5