Detailed information of TA system TA196764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2660792..2661394
• Replicon: chromosome
• Accession: NZ_CP071711
• Organism: Escherichia coli strain EC20017429

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JQM62_RS13190
• Protein ID: WP_000897305.1
• Coordinates: 2661083..2661394 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQM62_RS13185
• Protein ID: WP_000356397.1
• Coordinates: 2660792..2661082 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQM62_RS13160 (2656358)	2656358..2656993	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JQM62_RS13165 (2656990)	2656990..2657919	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JQM62_RS13170 (2658318)	2658318..2659298	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
JQM62_RS13175 (2659527)	2659527..2659769	-	243	WP_001086388.1	protein YiiF	-
JQM62_RS13180 (2659988)	2659988..2660206	-	219	WP_001295676.1	CopG family transcriptional regulator	-
JQM62_RS13185 (2660792)	2660792..2661082	-	291	WP_000356397.1	NadS family protein	Antitoxin
JQM62_RS13190 (2661083)	2661083..2661394	-	312	WP_000897305.1	hypothetical protein	Toxin
JQM62_RS13195 (2661623)	2661623..2662531	+	909	WP_001162704.1	alpha/beta hydrolase	-
JQM62_RS13200 (2662595)	2662595..2663536	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
JQM62_RS13205 (2663581)	2663581..2664018	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JQM62_RS13210 (2664015)	2664015..2664887	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JQM62_RS13215 (2664881)	2664881..2665480	-	600	WP_001295269.1	glucose-1-phosphatase	-
JQM62_RS13220 (2665579)	2665579..2666364	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2658318..2659298	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T196764 WP_000897305.1 NZ_CP071711:c2661394-2661083 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T196764 NZ_CP095407:1294079-1294261 [Acinetobacter pittii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATCAAGTTAGGGTTACAGGAAGTCATCATCACTT
CAAACACCCTACTAAGAAGGGACTGGTTACAGTTCCACATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAACAAGCAGGTCTTCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT196764 WP_000356397.1 NZ_CP071711:c2661082-2660792 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT196764 NZ_CP095407:1294351-1294755 [Acinetobacter pittii]
ATGTTATATCCGATTGCAATTGAAATGGGATCAGATACCGAGGCATTCGGTGTCATCGTTCCTGATATTCCCGGTTGCCA
CAGCGCCGGTGATACCCTCGAAGAAGCTCTCGATAATATTAAAGAAGCGATTGCAGGGCATTTAGAGATTCTTGCTGAAG
ATGGTGAAGAGATTCCTTTAGCTTCATCTGTAAGAAAATTTATGGATCTTCCTGAATATGATGGAATGGTTTGGTTCGTA
ACTGAGGTTGATTTAGGTCGTTATCTTGGAACCCCTGAAAAAATCAACGTATCTTTGCCTAGTCGTTTGATTCGTAAGAT
TGATGATAGTGTGGGTAAAGATAAGCAGTATAAAACCCGATCTGCATTTTTGGCTGCTGGTGCTGAAAAGCTGCTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7