Detailed information of TA system TA196676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4490228..4490982
• Replicon: chromosome
• Accession: NZ_CP071686
• Organism: Salmonella sp. SAL-007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: J1G46_RS21710
• Protein ID: WP_000558168.1
• Coordinates: 4490228..4490539 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J1G46_RS21715
• Protein ID: WP_001259009.1
• Coordinates: 4490536..4490982 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G46_RS21680	4485886..4486788	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
J1G46_RS21685	4486785..4487420	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
J1G46_RS21690	4487417..4488346	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
J1G46_RS21695	4488393..4488683	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
J1G46_RS21700	4488684..4488995	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
J1G46_RS21705	4489213..4490142	+	930	WP_001127705.1	alpha/beta hydrolase	-
J1G46_RS21710	4490228..4490539	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
J1G46_RS21715	4490536..4490982	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
J1G46_RS21720	4490997..4491938	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
J1G46_RS21725	4491983..4492420	-	438	WP_000560968.1	D-tyrosyl-tRNA(Tyr) deacylase	-
J1G46_RS21730	4492417..4493289	-	873	WP_000921423.1	virulence factor BrkB family protein	-
J1G46_RS21735	4493283..4493882	-	600	WP_000965695.1	glucose-1-phosphatase	-
J1G46_RS21740	4494073..4494876	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
J1G46_RS21745	4494910..4495806	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T196676 WP_000558168.1 NZ_CP071686:4490228-4490539 [Salmonella sp. SAL-007]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T196676 NZ_CP095253:2689375-2689743 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT196676 WP_001259009.1 NZ_CP071686:4490536-4490982 [Salmonella sp. SAL-007]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT196676 NZ_CP095253:2689157-2689378 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A1R2S541