Detailed information of TA system TA196675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4488393..4488995
• Replicon: chromosome
• Accession: NZ_CP071686
• Organism: Salmonella sp. SAL-007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: J1G46_RS21700
• Protein ID: WP_001159635.1
• Coordinates: 4488684..4488995 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J1G46_RS21695
• Protein ID: WP_000362050.1
• Coordinates: 4488393..4488683 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G46_RS21680	4485886..4486788	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
J1G46_RS21685	4486785..4487420	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
J1G46_RS21690	4487417..4488346	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
J1G46_RS21695	4488393..4488683	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
J1G46_RS21700	4488684..4488995	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
J1G46_RS21705	4489213..4490142	+	930	WP_001127705.1	alpha/beta hydrolase	-
J1G46_RS21710	4490228..4490539	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	-
J1G46_RS21715	4490536..4490982	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
J1G46_RS21720	4490997..4491938	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
J1G46_RS21725	4491983..4492420	-	438	WP_000560968.1	D-tyrosyl-tRNA(Tyr) deacylase	-
J1G46_RS21730	4492417..4493289	-	873	WP_000921423.1	virulence factor BrkB family protein	-
J1G46_RS21735	4493283..4493882	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T196675 WP_001159635.1 NZ_CP071686:c4488995-4488684 [Salmonella sp. SAL-007]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T196675 NZ_CP095253:c2281084-2280701 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT196675 WP_000362050.1 NZ_CP071686:c4488683-4488393 [Salmonella sp. SAL-007]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT196675 NZ_CP095253:c2281326-2281084 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97