Detailed information of TA system TA196612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 69950..70475
• Replicon: plasmid p2
• Accession: NZ_CP071614
• Organism: Rhizobium bangladeshense strain BLR175

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: J2J98_RS24205
• Protein ID: WP_207603680.1
• Coordinates: 70203..70475 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: J2J98_RS24200
• Protein ID: WP_064707332.1
• Coordinates: 69950..70201 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J98_RS24190	66466..67809	-	1344	WP_207603679.1	adenylate/guanylate cyclase domain-containing protein	-
J2J98_RS24195	68501..69838	+	1338	WP_064713262.1	nucleotide sugar dehydrogenase	-
J2J98_RS24200	69950..70201	+	252	WP_064707332.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
J2J98_RS24205	70203..70475	+	273	WP_207603680.1	Txe/YoeB family addiction module toxin	Toxin
J2J98_RS24210	70508..71680	-	1173	WP_207603681.1	DUF3095 family protein	-
J2J98_RS24215	71805..72284	+	480	WP_207603682.1	class I SAM-dependent methyltransferase	-
J2J98_RS24220	72381..72926	+	546	WP_138396148.1	GFA family protein	-
J2J98_RS24225	72901..73356	-	456	WP_064707328.1	Rrf2 family transcriptional regulator	-
J2J98_RS24230	73490..74395	+	906	WP_207603683.1	FAD-dependent oxidoreductase	-
J2J98_RS24235	74561..75001	+	441	WP_064707326.1	DUF805 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..461863	461863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10757.40 Da
• Isoelectric Point: 9.4680

>T196612 WP_207603680.1 NZ_CP071614:70203-70475 [Rhizobium bangladeshense]
MKLLWTPNSWEEYEYWQKTDQKLVEKINELLRDTKRSPFKGLGKPEPLKGDLSGFWSRRILGEHRLVYCVTGKGASQQLE
IIQCRFHYEK

Nucleotide

• Length: 273 bp

>T196612 NZ_CP095180:1744884-1745405 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATAATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGCGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGGGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGAATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGGCGCCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGTGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9289.53 Da
• Isoelectric Point: 5.6513

>AT196612 WP_064707332.1 NZ_CP071614:69950-70201 [Rhizobium bangladeshense]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIGQLRSGQGIEKD
PTL

Nucleotide

• Length: 252 bp

>AT196612 NZ_CP095180:1744608-1744877 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCCGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA