Detailed information of TA system TA196563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 890088..891331
• Replicon: chromosome
• Accession: NZ_CP071527
• Organism: Legionella lytica strain PCM 2298

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: J2N86_RS03960
• Protein ID: WP_252581108.1
• Coordinates: 890088..891026 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A378KXL1
• Locus tag: J2N86_RS03965
• Protein ID: WP_058474576.1
• Coordinates: 891023..891331 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2N86_RS03935 (J2N86_03950)	887662..887862	+	201	WP_252581106.1	DUF2892 domain-containing protein	-
J2N86_RS03940 (J2N86_03955)	887919..888353	+	435	WP_252581107.1	thioredoxin TrxC	-
J2N86_RS03945 (J2N86_03960)	888476..889534	+	1059	Protein_773	ACR3 family arsenite efflux transporter	-
J2N86_RS03950 (J2N86_03965)	889607..889702	+	96	Protein_774	ArsA-related P-loop ATPase	-
J2N86_RS03955	889911..890091	+	181	Protein_775	integrase	-
J2N86_RS03960 (J2N86_03975)	890088..891026	-	939	WP_252581108.1	lpg2370 family Dot/Icm T4SS effector	Toxin
J2N86_RS03965 (J2N86_03980)	891023..891331	-	309	WP_058474576.1	HipA N-terminal domain-containing protein	Antitoxin
J2N86_RS03970 (J2N86_03985)	891328..891555	-	228	WP_252581109.1	helix-turn-helix transcriptional regulator	-
J2N86_RS16105 (J2N86_03990)	891811..892110	-	300	WP_289781842.1	ATP-binding cassette domain-containing protein	-
J2N86_RS03980 (J2N86_03995)	892082..893317	-	1236	WP_252581110.1	MFS transporter	-
J2N86_RS03985 (J2N86_04000)	893525..894701	+	1177	Protein_781	ISL3 family transposase	-
J2N86_RS03990 (J2N86_04005)	895207..895902	+	696	WP_238584207.1	AbiEi antitoxin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-276)

Antitoxin

• HipA, N-terminal subdomain 1 (3-98)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35904.63 Da
• Isoelectric Point: 9.4483

>T196563 WP_252581108.1 NZ_CP071527:c891026-890088 [Legionella lytica]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLTAAEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTIAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYNS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKTSISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKNDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQPMQEKYLELLEQRCKRLNFFD

Nucleotide

• Length: 939 bp

>T196563 NZ_CP095162:c812118-811861 [Streptococcus suis]
TTGAGTTATAAGGTTATTTTGTCTGCTAAGGCCCAAAAGCAAATTCGAAAGATGGATAAAACCGCTGCAAGTTTGATTTT
GCGCTATCTCTACAAGCATATTGACGGTTGCGACAACCCTAGACAATACGGAAAAGCCTTGACTGCTAATCGTTCTGGCC
AATGGCGCTATCGTATCGGCAATTACAGGGTTATCGTTAATATTGAAGATGATAAATTGATTGTGACAAGCATAAAAATG
AGAAATGGAAGTATATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11487.29 Da
• Isoelectric Point: 4.4013

>AT196563 WP_058474576.1 NZ_CP071527:c891331-891023 [Legionella lytica]
MRKAYVSVNGIKAGILEELQGGAYQFTYFEDYQGAPVSLTMPLNNKIYDFGVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Nucleotide

• Length: 309 bp

>AT196563 NZ_CP095162:c812347-812108 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAGTACGATGCGCGTGTGGCAGACGTTGCTTGGGCAG
AATACCAAGCAGACTTGGCGAATAGAATTGAGCCGATGACATTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A378KXL1