Detailed information of TA system TA196552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3882477..3883171
• Replicon: chromosome
• Accession: NZ_CP071522
• Organism: Escherichia coli strain UPEC129

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: J3P56_RS19075
• Protein ID: WP_001263500.1
• Coordinates: 3882477..3882875 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: J3P56_RS19080
• Protein ID: WP_000554758.1
• Coordinates: 3882878..3883171 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P56_RS19050	3877842..3878300	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
J3P56_RS19055	3878561..3880018	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
J3P56_RS19060	3880075..3880689	-	615	WP_000602123.1	peptide chain release factor H	-
J3P56_RS19065	3880686..3881825	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
J3P56_RS19070	3882015..3882467	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
J3P56_RS19075	3882477..3882875	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J3P56_RS19080	3882878..3883171	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J3P56_RS19085	3883223..3884278	-	1056	WP_001226168.1	DNA polymerase IV	-
J3P56_RS19090	3884349..3885134	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
J3P56_RS19095	3885106..3886818	+	1713	Protein_3737	flagellar biosynthesis protein FlhA	-
J3P56_RS19100	3886897..3887055	+	159	WP_014639450.1	hypothetical protein	-
J3P56_RS19105	3887138..3887635	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3882477..3902831	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T196552 WP_001263500.1 NZ_CP071522:c3882875-3882477 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T196552 NZ_CP095156:c151228-150923 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT196552 WP_000554758.1 NZ_CP071522:c3883171-3882878 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT196552 NZ_CP095156:c151448-151230 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3