Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3655456..3656135 Replicon chromosome
Accession NZ_CP071522
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag J3P56_RS18015 Protein ID WP_000057524.1
Coordinates 3655833..3656135 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag J3P56_RS18010 Protein ID WP_000806442.1
Coordinates 3655456..3655797 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS18000 3651700..3652632 - 933 WP_000883052.1 glutaminase A -
J3P56_RS18005 3652894..3655398 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
J3P56_RS18010 3655456..3655797 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
J3P56_RS18015 3655833..3656135 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J3P56_RS18020 3656268..3657062 + 795 WP_000365177.1 TraB/GumN family protein -
J3P56_RS18025 3657266..3657745 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
J3P56_RS18030 3657769..3658569 + 801 WP_000439796.1 hypothetical protein -
J3P56_RS18035 3658566..3659069 + 504 WP_000667000.1 hypothetical protein -
J3P56_RS18040 3659107..3660759 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T196550 WP_000057524.1 NZ_CP071522:c3656135-3655833 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T196550 NZ_CP095155:c4490929-4490756 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT196550 WP_000806442.1 NZ_CP071522:c3655797-3655456 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT196550 NZ_CP095155:c4491253-4490957 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References