196540

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2374800..2375362 Replicon chromosome
Accession NZ_CP071522
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag J3P56_RS11470 Protein ID WP_000605675.1
Coordinates 2375084..2375362 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag J3P56_RS11465 Protein ID WP_000781370.1
Coordinates 2374800..2375084 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS11450 2369981..2373028 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
J3P56_RS11455 2373041..2373925 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
J3P56_RS11460 2373918..2374571 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
J3P56_RS11465 2374800..2375084 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
J3P56_RS11470 2375084..2375362 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J3P56_RS11475 2375548..2376558 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
J3P56_RS11480 2376692..2378389 - 1698 WP_000433462.1 malate dehydrogenase -
J3P56_RS11485 2378545..2378682 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
J3P56_RS11490 2378784..2378999 - 216 WP_000495766.1 biofilm-dependent modulation protein -
J3P56_RS11495 2379344..2379775 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T196540 WP_000605675.1 NZ_CP071522:c2375362-2375084 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T196540 NZ_CP095155:c2576506-2576330 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT196540 WP_000781370.1 NZ_CP071522:c2375084-2374800 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT196540 NZ_CP095155:c2576284-2575868 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References