Detailed information of TA system TA196531

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3993700..3994112
• Replicon: chromosome
• Accession: NZ_CP071521
• Organism: Escherichia coli strain 11202

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: J2D70_RS19535
• Protein ID: WP_000132601.1
• Coordinates: 3993771..3994112 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3993700..3993776 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2D70_RS19525 (3990563)	3990563..3992152	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
J2D70_RS19530 (3992149)	3992149..3993543	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3993700)	3993700..3993776	-	77	NuclAT_14	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_14	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_14	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_14	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_15	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_15	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_15	-	Antitoxin
- (3993700)	3993700..3993776	-	77	NuclAT_15	-	Antitoxin
J2D70_RS19535 (3993771)	3993771..3994112	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
J2D70_RS19540 (3994274)	3994274..3995653	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
J2D70_RS19545 (3995653)	3995653..3996699	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T196531 WP_000132601.1 NZ_CP071521:3993771-3994112 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T196531 NZ_CP095155:764943-765239 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT196531 NZ_CP071521:c3993776-3993700 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin