Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3679261..3679955 Replicon chromosome
Accession NZ_CP071521
Organism Escherichia coli strain 11202

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag J2D70_RS18055 Protein ID WP_001263489.1
Coordinates 3679261..3679659 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag J2D70_RS18060 Protein ID WP_000554758.1
Coordinates 3679662..3679955 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3674849) 3674849..3674929 - 81 NuclAT_12 - -
- (3674849) 3674849..3674929 - 81 NuclAT_12 - -
- (3674849) 3674849..3674929 - 81 NuclAT_12 - -
- (3674849) 3674849..3674929 - 81 NuclAT_12 - -
J2D70_RS18030 (3675525) 3675525..3675983 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
J2D70_RS18035 (3676244) 3676244..3677701 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
J2D70_RS18040 (3677758) 3677758..3678279 - 522 Protein_3552 peptide chain release factor H -
J2D70_RS18045 (3678275) 3678275..3678481 - 207 Protein_3553 RtcB family protein -
J2D70_RS18050 (3678799) 3678799..3679251 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
J2D70_RS18055 (3679261) 3679261..3679659 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
J2D70_RS18060 (3679662) 3679662..3679955 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
J2D70_RS18065 (3680007) 3680007..3681062 - 1056 WP_001226164.1 DNA polymerase IV -
J2D70_RS18070 (3681133) 3681133..3681918 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
J2D70_RS18075 (3681890) 3681890..3683602 + 1713 Protein_3559 flagellar biosynthesis protein FlhA -
J2D70_RS18080 (3683826) 3683826..3684323 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T196529 WP_001263489.1 NZ_CP071521:c3679659-3679261 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T196529 NZ_CP095155:220762-220869 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT196529 WP_000554758.1 NZ_CP071521:c3679955-3679662 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT196529 NZ_CP095155:c220714-220648 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References