Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 605937..606736 Replicon chromosome
Accession NZ_CP071521
Organism Escherichia coli strain 11202

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J2D70_RS02940 Protein ID WP_000347273.1
Coordinates 605937..606401 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J2D70_RS02945 Protein ID WP_001307405.1
Coordinates 606401..606736 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J2D70_RS02915 (602113) 602113..602670 - 558 Protein_574 amidohydrolase family protein -
J2D70_RS02920 (602666) 602666..603037 - 372 Protein_575 PTS sugar transporter subunit IIC -
J2D70_RS02925 (603048) 603048..603521 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
J2D70_RS02930 (603544) 603544..604824 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J2D70_RS02935 (605073) 605073..605882 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J2D70_RS02940 (605937) 605937..606401 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J2D70_RS02945 (606401) 606401..606736 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J2D70_RS02950 (606885) 606885..608456 - 1572 WP_001273753.1 galactarate dehydratase -
J2D70_RS02955 (608831) 608831..610165 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J2D70_RS02960 (610181) 610181..610951 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 594953..617611 22658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T196507 WP_000347273.1 NZ_CP071521:c606401-605937 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T196507 NZ_CP095145:4006470-4006688 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT196507 WP_001307405.1 NZ_CP071521:c606736-606401 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT196507 NZ_CP095145:4006069-4006443 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References