Detailed information of TA system TA196487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1193724..1194285
• Replicon: chromosome
• Accession: NZ_CP071491
• Organism: Glaesserella parasuis strain GHP1807

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: J1G54_RS06085
• Protein ID: WP_005712614.1
• Coordinates: 1193724..1194002 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: J1G54_RS06090
• Protein ID: WP_005712616.1
• Coordinates: 1194013..1194285 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G54_RS06080	1192565..1193575	-	1011	WP_207342894.1	ornithine carbamoyltransferase	-
J1G54_RS06085	1193724..1194002	+	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J1G54_RS06090	1194013..1194285	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
J1G54_RS06095	1194349..1195569	+	1221	WP_207342895.1	ATP-dependent RNA helicase RhlB	-
J1G54_RS06100	1195579..1197066	+	1488	WP_062923800.1	DUF853 domain-containing protein	-
J1G54_RS06105	1197220..1197762	-	543	WP_207342896.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T196487 WP_005712614.1 NZ_CP071491:1193724-1194002 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T196487 NZ_CP095137:4415027-4415404 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT196487 WP_005712616.1 NZ_CP071491:1194013-1194285 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT196487 NZ_CP095137:4414570-4414950 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: A0A143CE91