Detailed information of TA system TA196476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1058670..1059231
• Replicon: chromosome
• Accession: NZ_CP071490
• Organism: Glaesserella parasuis strain GHP1808

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B8F405
• Locus tag: J1G53_RS05005
• Protein ID: WP_012621703.1
• Coordinates: 1058953..1059231 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: J1G53_RS05000
• Protein ID: WP_005712616.1
• Coordinates: 1058670..1058942 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G53_RS04980	1055058..1055576	+	519	WP_075605585.1	single-stranded DNA-binding protein	-
J1G53_RS04985	1055782..1056996	+	1215	WP_112062944.1	ATP-binding protein	-
J1G53_RS04990	1057068..1057376	-	309	Protein_956	DUF853 family protein	-
J1G53_RS04995	1057386..1058606	-	1221	WP_207342590.1	ATP-dependent RNA helicase RhlB	-
J1G53_RS05000	1058670..1058942	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
J1G53_RS05005	1058953..1059231	-	279	WP_012621703.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J1G53_RS05010	1059380..1060390	+	1011	WP_005712612.1	ornithine carbamoyltransferase	-
J1G53_RS05015	1060737..1061999	+	1263	WP_012621704.1	serine hydroxymethyltransferase	-
J1G53_RS05020	1062113..1063441	+	1329	WP_160415963.1	exodeoxyribonuclease VII large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10384.74 Da
• Isoelectric Point: 9.6620

>T196476 WP_012621703.1 NZ_CP071490:c1059231-1058953 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQDNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T196476 NZ_CP095137:1971378-1971632 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT196476 WP_005712616.1 NZ_CP071490:c1058942-1058670 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT196476 NZ_CP095137:1971130-1971381 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: B8F405