Detailed information of TA system TA196461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1191773..1192334
• Replicon: chromosome
• Accession: NZ_CP071487
• Organism: Glaesserella parasuis strain YHP1818

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B8F405
• Locus tag: J1G47_RS05960
• Protein ID: WP_012621703.1
• Coordinates: 1192056..1192334 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: J1G47_RS05955
• Protein ID: WP_005712616.1
• Coordinates: 1191773..1192045 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G47_RS05935	1188137..1188679	+	543	WP_021115958.1	single-stranded DNA-binding protein	-
J1G47_RS05940	1188885..1190099	+	1215	WP_021114868.1	ATP-binding protein	-
J1G47_RS05945	1190171..1190479	-	309	Protein_1140	DUF853 family protein	-
J1G47_RS05950	1190489..1191709	-	1221	WP_207326499.1	ATP-dependent RNA helicase RhlB	-
J1G47_RS05955	1191773..1192045	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
J1G47_RS05960	1192056..1192334	-	279	WP_012621703.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J1G47_RS05965	1192481..1193491	+	1011	WP_005712612.1	ornithine carbamoyltransferase	-
J1G47_RS05970	1193838..1195100	+	1263	WP_075620774.1	serine hydroxymethyltransferase	-
J1G47_RS05975	1195214..1196542	+	1329	WP_207326500.1	exodeoxyribonuclease VII large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10384.74 Da
• Isoelectric Point: 9.6620

>T196461 WP_012621703.1 NZ_CP071487:c1192334-1192056 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQDNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T196461 NZ_CP095132:c4892829-4892545 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT196461 WP_005712616.1 NZ_CP071487:c1192045-1191773 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT196461 NZ_CP095132:c4893061-4892819 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: B8F405