Detailed information of TA system TA196430

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43396..43660
• Replicon: plasmid p2
• Accession: NZ_CP071443
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: J1F34_RS24175
• Protein ID: WP_001331364.1
• Coordinates: 43508..43660 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43396..43453 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS24160	39734..40804	-	1071	WP_000151579.1	IncI1-type conjugal transfer protein TrbB	-
J1F34_RS24165	40823..42031	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
J1F34_RS24170	42249..43235	-	987	WP_001257838.1	hypothetical protein	-
-	43396..43453	-	58	NuclAT_0	-	Antitoxin
-	43396..43453	-	58	NuclAT_0	-	Antitoxin
-	43396..43453	-	58	NuclAT_0	-	Antitoxin
-	43396..43453	-	58	NuclAT_0	-	Antitoxin
J1F34_RS24175	43508..43660	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
J1F34_RS24180	43732..43983	-	252	WP_001291964.1	hypothetical protein	-
J1F34_RS24185	44642..44818	-	177	WP_001054901.1	hypothetical protein	-
J1F34_RS24190	45150..45359	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
J1F34_RS24195	45431..46093	-	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
J1F34_RS24200	46158..48326	-	2169	WP_077250315.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88572	88572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T196430 WP_001331364.1 NZ_CP071443:43508-43660 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T196430 NZ_CP095125:c609621-609157 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT196430 NZ_CP071443:c43453-43396 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin