Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4026082..4026623 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag J1F34_RS19135 Protein ID WP_000615976.1
Coordinates 4026082..4026360 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag J1F34_RS19140 Protein ID WP_000729704.1
Coordinates 4026363..4026623 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS19120 4023664..4024242 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
J1F34_RS19125 4024448..4025215 + 768 WP_000333380.1 class II glutamine amidotransferase -
J1F34_RS19130 4025186..4025926 - 741 WP_001225679.1 murein L,D-transpeptidase -
J1F34_RS19135 4026082..4026360 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J1F34_RS19140 4026363..4026623 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J1F34_RS19145 4026809..4027582 + 774 WP_000543895.1 C40 family peptidase -
J1F34_RS19150 4027639..4027995 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
J1F34_RS19155 4027988..4028266 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
J1F34_RS19160 4028371..4030083 - 1713 Protein_3730 flagellar biosynthesis protein FlhA -
J1F34_RS19165 4030055..4030840 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 4010667..4028266 17599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T196424 WP_000615976.1 NZ_CP071441:c4026360-4026082 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T196424 NZ_CP095121:c4626485-4626201 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT196424 WP_000729704.1 NZ_CP071441:c4026623-4026363 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT196424 NZ_CP095121:c4626717-4626475 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References