Detailed information of TA system TA196418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3139216..3139818
• Replicon: chromosome
• Accession: NZ_CP071441
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: J1F34_RS15030
• Protein ID: WP_000897305.1
• Coordinates: 3139216..3139527 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J1F34_RS15035
• Protein ID: WP_000356397.1
• Coordinates: 3139528..3139818 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS15000	3134246..3135031	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
J1F34_RS15005	3135130..3135729	+	600	WP_001295269.1	glucose-1-phosphatase	-
J1F34_RS15010	3135723..3136595	+	873	WP_000920762.1	virulence factor BrkB family protein	-
J1F34_RS15015	3136592..3137029	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
J1F34_RS15020	3137074..3138015	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
J1F34_RS15025	3138079..3138987	-	909	WP_001162704.1	alpha/beta hydrolase	-
J1F34_RS15030	3139216..3139527	+	312	WP_000897305.1	hypothetical protein	Toxin
J1F34_RS15035	3139528..3139818	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
J1F34_RS15040	3140423..3140641	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
J1F34_RS15045	3140861..3141103	+	243	WP_001087409.1	hypothetical protein	-
J1F34_RS15050	3141433..3142362	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
J1F34_RS15055	3142359..3142994	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
J1F34_RS15060	3142991..3143893	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T196418 WP_000897305.1 NZ_CP071441:3139216-3139527 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T196418 NZ_CP095121:323848-324216 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT196418 WP_000356397.1 NZ_CP071441:3139528-3139818 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT196418 NZ_CP095121:323630-323851 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7