Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2326341..2327140 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag J1F34_RS11100 Protein ID WP_000347266.1
Coordinates 2326676..2327140 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J1F34_RS11095 Protein ID WP_001307405.1
Coordinates 2326341..2326676 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS11080 2322126..2322896 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
J1F34_RS11085 2322912..2324246 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J1F34_RS11090 2324621..2326192 + 1572 WP_001273741.1 galactarate dehydratase -
J1F34_RS11095 2326341..2326676 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J1F34_RS11100 2326676..2327140 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J1F34_RS11105 2327195..2328004 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J1F34_RS11110 2328253..2329533 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
J1F34_RS11115 2329556..2330029 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J1F34_RS11120 2330040..2330819 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
J1F34_RS11125 2330809..2331687 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
J1F34_RS11130 2331705..2332139 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2315466..2327140 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T196416 WP_000347266.1 NZ_CP071441:2326676-2327140 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T196416 NZ_CP095119:c2556640-2556533 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAGCAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT196416 WP_001307405.1 NZ_CP071441:2326341-2326676 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT196416 NZ_CP095119:2556456-2556516 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTATTGCAGTAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References