Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1145309..1145811 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag J1F34_RS05560 Protein ID WP_000767829.1
Coordinates 1145309..1145563 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J1F34_RS05565 Protein ID WP_001259255.1
Coordinates 1145560..1145811 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS05530 1140324..1140551 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
J1F34_RS05535 1140565..1141623 - 1059 WP_000492330.1 transport protein YeeE -
J1F34_RS05540 1141802..1143160 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
J1F34_RS05545 1143150..1143212 - 63 WP_010723108.1 membrane protein YoeI -
J1F34_RS05550 1143427..1144356 - 930 WP_000803351.1 LysR family transcriptional regulator -
J1F34_RS05555 1144402..1145226 - 825 WP_000754737.1 SDR family oxidoreductase -
J1F34_RS05560 1145309..1145563 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J1F34_RS05565 1145560..1145811 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J1F34_RS05570 1146095..1146145 + 51 WP_001364200.1 his operon leader peptide -
J1F34_RS05575 1146291..1147190 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
J1F34_RS05580 1147196..1148500 + 1305 WP_001446927.1 histidinol dehydrogenase -
J1F34_RS05585 1148497..1149567 + 1071 WP_000108933.1 histidinol-phosphate transaminase -
J1F34_RS05590 1149567..1150634 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T196412 WP_000767829.1 NZ_CP071441:c1145563-1145309 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T196412 NZ_CP095119:c2198767-2198405 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT196412 WP_001259255.1 NZ_CP071441:c1145811-1145560 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT196412 NZ_CP095119:c2198934-2198764 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References