Detailed information of TA system TA196384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3903825..3904519
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: V0YKG6
• Locus tag: J1F19_RS18360
• Protein ID: WP_001263485.1
• Coordinates: 3904121..3904519 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: J1F19_RS18355
• Protein ID: WP_000554758.1
• Coordinates: 3903825..3904118 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS18335	3899242..3899739	+	498	WP_000006263.1	REP-associated tyrosine transposase RayT	-
J1F19_RS18340	3900178..3901890	-	1713	Protein_3578	flagellar biosynthesis protein FlhA	-
J1F19_RS18345	3901862..3902647	+	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
J1F19_RS18350	3902718..3903773	+	1056	WP_001226177.1	DNA polymerase IV	-
J1F19_RS18355	3903825..3904118	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J1F19_RS18360	3904121..3904519	+	399	WP_001263485.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J1F19_RS18365	3904529..3904981	+	453	WP_001059899.1	GNAT family N-acetyltransferase	-
J1F19_RS18370	3905227..3905433	+	207	Protein_3584	RtcB family protein	-
J1F19_RS18375	3905429..3905781	+	353	Protein_3585	peptide chain release factor H	-
J1F19_RS18380	3905838..3907295	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
J1F19_RS18385	3907556..3908014	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J1F19_RS18390	3908106..3909350	+	1245	WP_001586695.1	esterase FrsA	-
-	3908610..3908690	+	81	NuclAT_12	-	-
-	3908610..3908690	+	81	NuclAT_12	-	-
-	3908610..3908690	+	81	NuclAT_12	-	-
-	3908610..3908690	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15418.84 Da
• Isoelectric Point: 7.3840

>T196384 WP_001263485.1 NZ_CP071439:3904121-3904519 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T196384 NZ_CP095109:c2527564-2527298 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGGTTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCGTTATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT196384 WP_000554758.1 NZ_CP071439:3903825-3904118 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT196384 NZ_CP095109:c2527815-2527564 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATATGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAAGATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACATTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: V0YKG6

Antitoxin

• AlphaFold DB: S1QAE3