Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2189464..2190263 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag J1F19_RS10395 Protein ID WP_000347272.1
Coordinates 2189799..2190263 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J1F19_RS10390 Protein ID WP_001307405.1
Coordinates 2189464..2189799 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS10375 2185249..2186019 - 771 WP_207291711.1 2-dehydro-3-deoxyglucarate aldolase -
J1F19_RS10380 2186035..2187369 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
J1F19_RS10385 2187744..2189315 + 1572 WP_001273772.1 galactarate dehydratase -
J1F19_RS10390 2189464..2189799 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J1F19_RS10395 2189799..2190263 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J1F19_RS10400 2190318..2191127 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
J1F19_RS10405 2191376..2192656 + 1281 WP_000681955.1 tagatose-bisphosphate aldolase subunit KbaZ -
J1F19_RS10410 2192679..2193152 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J1F19_RS10415 2193163..2193942 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
J1F19_RS10420 2193932..2194810 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
J1F19_RS10425 2194828..2195262 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2180102..2190263 10161


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T196376 WP_000347272.1 NZ_CP071439:2189799-2190263 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T196376 NZ_CP095107:901668-901889 [Staphylococcus delphini]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGAGTGTTGTTATTGATTTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCATATAAAGGAGTGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT196376 WP_001307405.1 NZ_CP071439:2189464-2189799 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT196376 NZ_CP095107:901368-901655 [Staphylococcus delphini]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAG
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGTTGCATCATGCATAAGATTGACGCAGGGCACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACGTTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References