196375

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2146371..2147098 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag J1F19_RS10175 Protein ID WP_000550189.1
Coordinates 2146784..2147098 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag J1F19_RS10170 Protein ID WP_000560253.1
Coordinates 2146371..2146787 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS10160 2141786..2144137 + 2352 WP_000695464.1 alpha-glucosidase -
J1F19_RS10165 2144308..2146326 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
J1F19_RS10170 2146371..2146787 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J1F19_RS10175 2146784..2147098 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
J1F19_RS10180 2147383..2148519 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
J1F19_RS10185 2148604..2149107 + 504 WP_001309773.1 M48 family metallopeptidase -
J1F19_RS10190 2149184..2149867 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
J1F19_RS10195 2149946..2150932 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T196375 WP_000550189.1 NZ_CP071439:c2147098-2146784 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T196375 NZ_CP095104:c1814215-1814009 [Staphylococcus simulans]
ATGCATAGTAGTGTGATTCAAATCAGTGGTATGGAAACTGAAGAACAACAGCATATTTTGCAAGAACATTTGCAGCAAAT
GATTGGTATGTCAGAAGTGCAAGTGGATCTGAAATCACAAGAAGTGAAAGCGAGTTACCAGACACCAATCAGTTTAAACA
ATTTGGAAAAAGAAATTTATGATCAAGGTTACCAAATATTAAATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT196375 WP_000560253.1 NZ_CP071439:c2146787-2146371 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT196375 NZ_CP095104:c1814523-1814230 [Staphylococcus simulans]
ATGACGCAAGAAGAACGCGCACATCATTCAGAAAAAATCAAATCTAACCTTAAAGCACGACTGAATCGCATAGAAGGACA
AGTCAGAGCGATTAATCGCATGATAGATGAAGATGTTTATTGTGATGATGTTTTAACACAAATCAGAGCGACACGTTCAG
CTTTGAATAGTGTTGCTGTAAAATTGCTGGATCATCATATGAAAGGCTGTATTTTGCATAAAGCTGAAGATGGACAAGCA
GAAGCTGCAATGGCAGAATTATTAGTTACCGTCCAAAAATTAATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References