Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 267497..268059 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag J1F19_RS01355 Protein ID WP_000605676.1
Coordinates 267781..268059 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag J1F19_RS01350 Protein ID WP_000781370.1
Coordinates 267497..267781 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS01335 262679..265726 + 3048 WP_077577597.1 formate dehydrogenase-N subunit alpha -
J1F19_RS01340 265739..266623 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
J1F19_RS01345 266616..267269 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
J1F19_RS01350 267497..267781 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
J1F19_RS01355 267781..268059 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J1F19_RS01360 268245..269255 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
J1F19_RS01365 269389..271086 - 1698 WP_000433464.1 malate dehydrogenase -
J1F19_RS01370 271243..271380 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
J1F19_RS01375 271482..271697 - 216 WP_000495766.1 biofilm-dependent modulation protein -
J1F19_RS01380 272042..272473 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T196363 WP_000605676.1 NZ_CP071439:c268059-267781 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T196363 NZ_CP095095:c3509-3108 [Flavonifractor plautii]
ATGACCTATATGTTGGACACAAATATCTGCATCTATTCTATGAAGAAAAAGCCGGAAAAGGTGTTGAACAGGTTCCGGGA
GGAGTTGGACGGAGGCCTCTGCATTTCCTCTATCACTCTGGCCGAGTTGGAGTATGGGATGAAACACAGCTCTGACCCGG
TAAGAAATGAGCAGGCCCTTTTGCGCTTTCTGGCTCCGTTGAGCATCCTGCCCTTTGGGGCGGGGGCGGCGGCGGAGTAT
GGAGAGATCCGGGCTTATTTACAGAGCCGGGGAACGCCGATCGGCCCGCTGGATATGCTGATTGCTGCCCATGCACGTGC
AGAAGGAATAATCCTGGTAACGAATAATGTACGGGAGTTTGAGCGCGTGCCGGATCTGGAACTTGAAAACTGGGCGGAGT
AA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT196363 WP_000781370.1 NZ_CP071439:c267781-267497 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT196363 NZ_CP095095:c2982-2710 [Flavonifractor plautii]
ATGGCTAACACAAATATCAATATTCGTATGGATACAGACTTGAAAAAGCAGTTTGAAGCGTTTTGCGCAGACATGGGAAT
GACTATGACAACGGCATTTAATGTTTTTGCGAGAAAGGCTGTCCGAGAGTATAGAATACCATTTGAAGTTGGCGGAGAGG
TACCGAATGACGACACTAAAAAGGCTATGGAGGACGCGCAAAAAGGGATCGGCATGAGCAAGGGGTTTTCTTCTGTAGAA
GAACTCATGGAGGAGTTAAATGCTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References