Detailed information of TA system TA196358

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 123732..124103
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: J1F19_RS00630
• Protein ID: WP_001317028.1
• Coordinates: 123909..124103 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	123732..123910 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS00605	119687..121060	+	1374	WP_000123754.1	ATP-dependent RNA helicase DbpA	-
J1F19_RS00610	121189..122124	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
J1F19_RS00615	122176..123411	-	1236	WP_000040858.1	site-specific integrase	-
J1F19_RS00620	123413..123628	-	216	WP_000079604.1	excisionase XisR	-
J1F19_RS00625	123707..123916	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	123732..123910	+	179	NuclAT_0	-	Antitoxin
-	123732..123910	+	179	NuclAT_0	-	Antitoxin
-	123732..123910	+	179	NuclAT_0	-	Antitoxin
-	123732..123910	+	179	NuclAT_0	-	Antitoxin
J1F19_RS00630	123909..124103	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
J1F19_RS00635	124160..124969	-	810	WP_000166319.1	recombination protein RecT	-
J1F19_RS00640	124962..127562	-	2601	WP_001586992.1	exodeoxyribonuclease VIII	-
J1F19_RS00645	127664..127939	-	276	WP_000632297.1	protein RacC	-
J1F19_RS00650	128014..128184	-	171	WP_001352098.1	conserved protein, Rac prophage	-
J1F19_RS00655	128184..128405	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	122176..169728	47552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T196358 WP_001317028.1 NZ_CP071439:c124103-123909 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T196358 NZ_CP095094:c591161-590823 [Flavonifractor plautii]
ATGGTGACGATTTTTGAACAGGGTGATATCGTCTATCTGGACTTTGATCCCCAGTCCGGCCATGAGCAGAAGGGCCGCCG
TCCTGCCTTGGTGATCAGCAACAACTTGTTCAACCGGGTCAGCAGCCTGACCATGGTTTGCCCCATCACCCACACAGACC
GGGGCCACCCGTTCCACCTCCGGCTGGATGAGCGTACCAGGACGGACGGGGTCATCCTGTGTGATCAGGCCCGGATGCTG
GATCTGAACAGCCGCCATGCCTCCTTTGTAGAGCGTGCTCCGGAGGATATCTCCTCAGAGGCGGTAGATATGGTCATAGG
ATTCCTGGAGCCGAAGTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT196358 NZ_CP071439:123732-123910 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin