Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3574345..3574886 Replicon chromosome
Accession NZ_CP071436
Organism Escherichia coli strain EcPNK006

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag J1137_RS17360 Protein ID WP_000615979.1
Coordinates 3574608..3574886 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag J1137_RS17355 Protein ID WP_000729704.1
Coordinates 3574345..3574605 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1137_RS17335 3570028..3570813 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
J1137_RS17340 3570785..3572497 + 1713 Protein_3391 flagellar biosynthesis protein FlhA -
J1137_RS17345 3572713..3573210 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
J1137_RS17350 3573386..3574159 - 774 WP_133296758.1 C40 family peptidase -
J1137_RS17355 3574345..3574605 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J1137_RS17360 3574608..3574886 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J1137_RS17365 3575042..3575782 + 741 WP_001225679.1 murein L,D-transpeptidase -
J1137_RS17370 3575753..3576520 - 768 WP_000333379.1 class II glutamine amidotransferase -
J1137_RS17375 3576726..3577304 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T196352 WP_000615979.1 NZ_CP071436:3574608-3574886 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T196352 NZ_CP095090:846423-846785 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT196352 WP_000729704.1 NZ_CP071436:3574345-3574605 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT196352 NZ_CP095090:846256-846426 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References