Detailed information of TA system TA196351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3568156..3568850
• Replicon: chromosome
• Accession: NZ_CP071436
• Organism: Escherichia coli strain EcPNK006

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: J1137_RS17320
• Protein ID: WP_001263493.1
• Coordinates: 3568156..3568554 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: J1137_RS17325
• Protein ID: WP_000554757.1
• Coordinates: 3568557..3568850 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1137_RS17290	3563156..3564400	-	1245	WP_000189541.1	esterase FrsA	-
-	3563816..3563896	-	81	NuclAT_12	-	-
-	3563816..3563896	-	81	NuclAT_12	-	-
-	3563816..3563896	-	81	NuclAT_12	-	-
-	3563816..3563896	-	81	NuclAT_12	-	-
J1137_RS17295	3564492..3564950	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J1137_RS17300	3565211..3566668	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
J1137_RS17305	3566725..3567246	-	522	Protein_3384	peptide chain release factor H	-
J1137_RS17310	3567245..3567448	-	204	Protein_3385	RNA ligase RtcB family protein	-
J1137_RS17315	3567694..3568146	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
J1137_RS17320	3568156..3568554	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J1137_RS17325	3568557..3568850	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J1137_RS17330	3568902..3569957	-	1056	WP_001226164.1	DNA polymerase IV	-
J1137_RS17335	3570028..3570813	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
J1137_RS17340	3570785..3572497	+	1713	Protein_3391	flagellar biosynthesis protein FlhA	-
J1137_RS17345	3572713..3573210	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T196351 WP_001263493.1 NZ_CP071436:c3568554-3568156 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T196351 NZ_CP095090:835371-835580 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT196351 WP_000554757.1 NZ_CP071436:c3568850-3568557 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT196351 NZ_CP095090:835071-835364 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6