Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1749788..1750290 Replicon chromosome
Accession NZ_CP071436
Organism Escherichia coli strain EcPNK006

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag J1137_RS08275 Protein ID WP_000767829.1
Coordinates 1750036..1750290 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J1137_RS08270 Protein ID WP_001259255.1
Coordinates 1749788..1750039 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1137_RS08245 1744965..1746032 - 1068 WP_074579688.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
J1137_RS08250 1746032..1747102 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
J1137_RS08255 1747099..1748403 - 1305 WP_087898060.1 histidinol dehydrogenase -
J1137_RS08260 1748409..1749308 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
J1137_RS08265 1749454..1749504 - 51 WP_001364200.1 his operon leader peptide -
J1137_RS08270 1749788..1750039 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J1137_RS08275 1750036..1750290 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J1137_RS08280 1750373..1751197 + 825 WP_000754737.1 SDR family oxidoreductase -
J1137_RS08285 1751243..1752172 + 930 WP_000803366.1 LysR family transcriptional regulator -
J1137_RS08290 1752387..1752449 + 63 WP_010723108.1 membrane protein YoeI -
J1137_RS08295 1752439..1753797 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
J1137_RS08300 1753976..1755034 + 1059 WP_000492330.1 transport protein YeeE -
J1137_RS08305 1755048..1755275 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T196339 WP_000767829.1 NZ_CP071436:1750036-1750290 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T196339 NZ_CP095088:2672917-2673024 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT196339 WP_001259255.1 NZ_CP071436:1749788-1750039 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT196339 NZ_CP095088:c2672870-2672804 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References