Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 598185..598984 Replicon chromosome
Accession NZ_CP071436
Organism Escherichia coli strain EcPNK006

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag J1137_RS02895 Protein ID WP_000347251.1
Coordinates 598185..598649 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J1137_RS02900 Protein ID WP_001307405.1
Coordinates 598649..598984 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1137_RS02865 593186..593620 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
J1137_RS02870 593638..594516 - 879 Protein_562 PTS mannose/fructose/sorbose transporter family subunit IID -
J1137_RS02875 594506..595285 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
J1137_RS02880 595296..595769 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J1137_RS02885 595792..597072 - 1281 Protein_565 tagatose-bisphosphate aldolase subunit KbaZ -
J1137_RS02890 597321..598130 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J1137_RS02895 598185..598649 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J1137_RS02900 598649..598984 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J1137_RS02905 599133..600704 - 1572 WP_001273753.1 galactarate dehydratase -
J1137_RS02910 601079..602413 + 1335 WP_074551609.1 galactarate/glucarate/glycerate transporter GarP -
J1137_RS02915 602429..603199 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T196334 WP_000347251.1 NZ_CP071436:c598649-598185 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T196334 NZ_CP095088:1817947-1818201 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT196334 WP_001307405.1 NZ_CP071436:c598984-598649 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT196334 NZ_CP095088:1817699-1817950 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References