Detailed information of TA system TA196321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 194575..195293
• Replicon: plasmid Plas2
• Accession: NZ_CP071407
• Organism: Pantoea deleyi strain LMG24200

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J1C59_RS20190
• Protein ID: WP_128084768.1
• Coordinates: 194575..194883 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J1C59_RS20195
• Protein ID: WP_128084769.1
• Coordinates: 194883..195293 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1C59_RS20175	190036..191136	+	1101	WP_128084765.1	site-specific integrase	-
J1C59_RS20180	191140..191712	-	573	WP_128084766.1	lipid IV(A) palmitoyltransferase PagP	-
J1C59_RS20185	192159..194372	+	2214	WP_128084767.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
J1C59_RS20190	194575..194883	+	309	WP_128084768.1	type II toxin-antitoxin system HigB family toxin	Toxin
J1C59_RS20195	194883..195293	+	411	WP_128084769.1	helix-turn-helix domain-containing protein	Antitoxin
J1C59_RS20200	195398..196243	-	846	WP_128084770.1	VOC family protein	-
J1C59_RS20205	196264..197403	-	1140	WP_128084771.1	amidohydrolase	-
J1C59_RS20210	197415..198506	-	1092	WP_128084772.1	alkylhydroperoxidase domain protein	-
J1C59_RS20215	198503..199510	-	1008	WP_128084773.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..450383	450383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-134)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12202.21 Da
• Isoelectric Point: 10.2580

>T196321 WP_128084768.1 NZ_CP071407:194575-194883 [Pantoea deleyi]
MHVISRVPFDEAVKKHPKAAAALELLYRNLRQGDFRTPEDLRALYASLDRMKYRARWWVIDLSGNQIRVMFFADFERSKI
FIKHICTHAEYDKLVKKYREAT

Nucleotide

• Length: 309 bp

>T196321 NZ_CP095086:c4684858-4684685 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15175.20 Da
• Isoelectric Point: 4.5425

>AT196321 WP_128084769.1 NZ_CP071407:194883-195293 [Pantoea deleyi]
MNSEAIRLSNQLAEALPLLAGSGQQRDYEDALELVEYLIEHAPDNPLVDLLCARIADYEDNDPGLAIFNARQREIESGSA
VLRVLIEQHNLRLSDFENEIGKKSLVSRILSGERQLTVAHIRALCARFGLSPSLFF

Nucleotide

• Length: 411 bp

>AT196321 NZ_CP095086:c4685182-4684886 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG