Detailed information of TA system TA196316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 268285..268831
• Replicon: plasmid p2
• Accession: NZ_CP071401
• Organism: Rhizobium leguminosarum strain OyaliB

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: J0664_RS30745
• Protein ID: WP_207159696.1
• Coordinates: 268538..268831 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: J0664_RS30740
• Protein ID: WP_168259268.1
• Coordinates: 268285..268536 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0664_RS30730	264932..266275	-	1344	WP_168344288.1	adenylate/guanylate cyclase domain-containing protein	-
J0664_RS30735	266819..268156	+	1338	WP_168339675.1	nucleotide sugar dehydrogenase	-
J0664_RS30740	268285..268536	+	252	WP_168259268.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
J0664_RS30745	268538..268831	+	294	WP_207159696.1	Txe/YoeB family addiction module toxin	Toxin
J0664_RS30750	268864..270090	-	1227	WP_077979945.1	DUF3095 domain-containing protein	-
J0664_RS30755	270264..270743	+	480	WP_207159472.1	class I SAM-dependent methyltransferase	-
J0664_RS30760	270871..272661	-	1791	WP_207159473.1	ATP-binding cassette domain-containing protein	-
J0664_RS30765	273005..273460	-	456	WP_207159474.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..538428	538428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11711.44 Da
• Isoelectric Point: 8.6839

>T196316 WP_207159696.1 NZ_CP071401:268538-268831 [Rhizobium leguminosarum]
MKLQWTPNSWEEYEYWQKNDQKMVERIDELLKDTKRSPFKGLGKPEPLKGDLYGFWSRRILGEHRLVYCVTGKGSNQQLE
IIQCRFHYEKWVLAQCS

Nucleotide

• Length: 294 bp

>T196316 NZ_CP095086:3919851-3920507 [Escherichia coli]
ATGGCCTATGACTACAGCGCCAGCCTCAACCCGCAAAAAGCCCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGGAACGTGGCAGCACACGACGTGCCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGAATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAGCAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9273.53 Da
• Isoelectric Point: 5.6513

>AT196316 WP_168259268.1 NZ_CP071401:268285-268536 [Rhizobium leguminosarum]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLVAEGEYESMQETLHLLSTPANSARLRESLGQLRKGQVVESD
PTL

Nucleotide

• Length: 252 bp

>AT196316 NZ_CP095086:3920504-3921571 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCTTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTAAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAAAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGA
CTGCACCAGTGGCCTGCGGGCAAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA