Detailed information of TA system TA196284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 50665..51392
• Replicon: plasmid unnamed1
• Accession: NZ_CP071386
• Organism: Klebsiella pneumoniae strain wxc2020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: J1F26_RS26065
• Protein ID: WP_011251285.1
• Coordinates: 50665..50976 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J1F26_RS26070
• Protein ID: WP_011251286.1
• Coordinates: 50973..51392 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F26_RS26040	47431..47610	-	180	Protein_50	IS5/IS1182 family transposase	-
J1F26_RS26045	47650..49178	-	1529	Protein_51	IS66 family transposase	-
J1F26_RS26050	49227..49574	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
J1F26_RS26055	49571..49909	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
J1F26_RS26060	50002..50460	+	459	Protein_54	DDE-type integrase/transposase/recombinase	-
J1F26_RS26065	50665..50976	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
J1F26_RS26070	50973..51392	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
J1F26_RS26075	51539..52507	+	969	WP_077268729.1	IS5 family transposase	-
J1F26_RS26080	52576..53111	-	536	Protein_58	transposase	-
J1F26_RS26085	53636..55003	-	1368	WP_032437385.1	formimidoylglutamate deiminase	-
J1F26_RS26090	55106..55867	+	762	WP_017900871.1	histidine utilization repressor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / mph(A) / blaDHA-1 / qnrB4 / aph(3'')-Ib / aph(6)-Id / sul2	-	1..195272	195272
	-	inside	IScluster/Tn	-	-	42741..52507	9766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T196284 WP_011251285.1 NZ_CP071386:50665-50976 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T196284 NZ_CP095083:684592-684906 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT196284 WP_011251286.1 NZ_CP071386:50973-51392 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT196284 NZ_CP095083:684903-685319 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1