Detailed information of TA system TA196237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4344749..4345548
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: J0O73_RS20965
• Protein ID: WP_048969505.1
• Coordinates: 4345084..4345548 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: J0O73_RS20960
• Protein ID: WP_001307405.1
• Coordinates: 4344749..4345084 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS20945	4340534..4341304	-	771	WP_024250085.1	2-dehydro-3-deoxyglucarate aldolase	-
J0O73_RS20950	4341320..4342654	-	1335	WP_000599633.1	galactarate/glucarate/glycerate transporter GarP	-
J0O73_RS20955	4343029..4344600	+	1572	WP_001273763.1	galactarate dehydratase	-
J0O73_RS20960	4344749..4345084	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
J0O73_RS20965	4345084..4345548	+	465	WP_048969505.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
J0O73_RS20970	4345603..4346412	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
J0O73_RS20975	4346661..4347941	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
J0O73_RS20980	4347964..4348437	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
J0O73_RS20985	4348448..4349227	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
J0O73_RS20990	4349217..4350095	+	879	WP_137505491.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
J0O73_RS20995	4350113..4350547	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17862.33 Da
• Isoelectric Point: 9.6924

>T196237 WP_048969505.1 NZ_CP071375:4345084-4345548 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFIKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T196237 NZ_CP095023:c1943333-1942944 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT196237 WP_001307405.1 NZ_CP071375:4344749-4345084 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT196237 NZ_CP095023:c1943557-1943330 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7