Detailed information of TA system TA196231

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3058019..3058244
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: J0O73_RS14925
• Protein ID: WP_000813255.1
• Coordinates: 3058019..3058174 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3058186..3058244 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS14875	3053770..3054025	-	256	Protein_2899	DUF3927 family protein	-
J0O73_RS14880	3054022..3054450	-	429	Protein_2900	tellurite resistance TerB family protein	-
J0O73_RS14900	3055084..3055773	-	690	WP_001064889.1	antiterminator	-
J0O73_RS14905	3055770..3056129	-	360	WP_001217464.1	RusA family crossover junction endodeoxyribonuclease	-
J0O73_RS14910	3056142..3057191	-	1050	WP_001265301.1	DUF968 domain-containing protein	-
J0O73_RS14915	3057193..3057471	-	279	WP_001360223.1	hypothetical protein	-
J0O73_RS14920	3057538..3057801	-	264	WP_000975572.1	hypothetical protein	-
J0O73_RS14925	3058019..3058174	-	156	WP_000813255.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3058186..3058244	+	59	-	-	Antitoxin
J0O73_RS14930	3058917..3059612	+	696	WP_000892866.1	hypothetical protein	-
J0O73_RS14935	3059625..3060278	+	654	WP_001373963.1	class I SAM-dependent methyltransferase	-
J0O73_RS14940	3060593..3061492	-	900	WP_000566848.1	SMEK domain-containing protein	-
J0O73_RS14945	3061745..3062167	-	423	WP_001151251.1	DUF977 family protein	-
J0O73_RS14950	3062208..3063173	-	966	WP_000054501.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleB2 / nleB2	3021900..3085028	63128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5726.85 Da
• Isoelectric Point: 6.1531

>T196231 WP_000813255.1 NZ_CP071375:c3058174-3058019 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYESEE

Nucleotide

• Length: 156 bp

>T196231 NZ_CP095023:1386500-1386931 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT196231 NZ_CP071375:3058186-3058244 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin