Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2514848..2515410 Replicon chromosome
Accession NZ_CP071375
Organism Escherichia coli strain E105

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag J0O73_RS12035 Protein ID WP_000605676.1
Coordinates 2515132..2515410 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag J0O73_RS12030 Protein ID WP_000781370.1
Coordinates 2514848..2515132 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0O73_RS12015 2510207..2513254 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
J0O73_RS12020 2513267..2514151 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
J0O73_RS12025 2514144..2514797 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
J0O73_RS12030 2514848..2515132 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
J0O73_RS12035 2515132..2515410 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J0O73_RS12040 2515596..2516606 - 1011 WP_000642406.1 alcohol dehydrogenase AdhP -
J0O73_RS12045 2516740..2518437 - 1698 WP_000433464.1 malate dehydrogenase -
J0O73_RS12050 2518594..2518731 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
J0O73_RS12055 2518833..2519048 - 216 WP_096220623.1 biofilm-dependent modulation protein -
J0O73_RS12060 2519393..2519824 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T196222 WP_000605676.1 NZ_CP071375:c2515410-2515132 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T196222 NZ_CP095023:719582-719989 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT196222 WP_000781370.1 NZ_CP071375:c2515132-2514848 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT196222 NZ_CP095023:719325-719585 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References